Xrcc5^tm1Dbr
Targeted Allele Detail

Summary

• Symbol: Xrcc5^tm1Dbr
• Name: X-ray repair complementing defective repair in Chinese hamster cells 5; targeted mutation 1, David B Roth
• MGI ID: MGI:2179987
• Synonyms: Ku80-, Ku86-, xrcc5-, xrcc5^M1
• Gene: Xrcc5 Location: Chr1:72346586-72434111 bp, + strand Genetic Position: Chr1, 36.5 cM, cytoband E
• Alliance: Xrcc5^tm1Dbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34834
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 6 kb of this gene encoding amino acids 229-313 were replaced with a PGK-puro cassette via homologous recombination resulting in a frameshift after residue 313 and deleting 69% of the protein product. Northern blot analysis and RT-PCR of embryonic fibroblasts from homozygous mutants did not detect gene transcript. (J:34834)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Xrcc5^tm1Dbr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xrcc5 Mutation: 71 strains or lines available

References

• Original: J:34834 Zhu C, et al., Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. Cell. 1996 Aug 9;86(3):379-89
• All: 25 reference(s)