Ckm^tm1Bew
Targeted Allele Detail

Summary

• Symbol: Ckm^tm1Bew
• Name: creatine kinase, muscle; targeted mutation 1, Be Wieringa
• MGI ID: MGI:2179990
• Synonyms: M-CK-, MM-CK-, MMKO
• Gene: Ckm Location: Chr7:19145019-19155508 bp, + strand Genetic Position: Chr7, 9.67 cM
• Alliance: Ckm^tm1Bew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76699
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.9 kb region encompassing exon 2 and part of introns 1 and 2 was replaced with a hygroB resistance cassette via homologous recombination. Northern blot using an exon 2 probe demonstrated lack of gene transcripts in skeletal muscle and staining for enzyme activity was negative in homogenates of skeletal and cardiac muscle from homozygous mutant animals. (J:76699)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ckm Mutation: 38 strains or lines available

References

• Original: J:76699 van Deursen J, et al., Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity. Cell. 1993 Aug 27;74(4):621-31
• All: 34 reference(s)