Tg(tetO-Kras2)12Hev
Transgene Detail

Summary

• Symbol: Tg(tetO-Kras2)12Hev
• Name: transgene insertion 12, Harold E Varmus
• MGI ID: MGI:2179992
• Synonyms: KE, K-ras4bG12D, K-Ras4b^G12D responder, K-Ras4bG12D responder, K-rasG12D-5, TetO-Kras^G12D, Tet-op-K-ras, Tet-op-K-Ras^G12D, TOR, tre-Kras, TRE-KrasG12D
• Transgene: Tg(tetO-Kras2)12Hev Location: unknown
• Alliance: Tg(tetO-Kras2)12Hev page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Kras2)12Hev expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Kras (24525)

  Kras	(MGI:96680)

• Mutation details: The transgene expresses the mutant gene Kras4b^G12D, under the control of a tetracycline-responsive promoter element (tTA), mp-1 intron polyadenylation site sequence, and a minimal CMV promoter. (J:73468)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(tetO-Kras2)12Hev

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Homozygous transgenic mice are viable and fertile. In conjunction with a second transgene encoding either rtTA (reverse tetracycline trans-activator protein) or tTA (the tetracycline trans-activator), mice carrying Tg(tetO-Kras2)12Hev show tissue-specific expression of the mutated K-ras transgene that may be induced or suppressed by administration of the tetracycline analog, doxycycline. Cessation or initiation of doxycycline treatment in bitransgenic strains containing, respectively, rtTA or tTA, will result in a rapid decrease of expression of the transgene.

References

• Original: J:73468 Fisher GH, et al., Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes. Genes Dev. 2001 Dec 15;15(24):3249-62
• All: 67 reference(s)