Ckm^tm2Bew
Targeted Allele Detail

Summary

• Symbol: Ckm^tm2Bew
• Name: creatine kinase, muscle; targeted mutation 2, Be Wieringa
• MGI ID: MGI:2179994
• Synonyms: M-CK^I
• Gene: Ckm Location: Chr7:19145019-19155508 bp, + strand Genetic Position: Chr7, 9.67 cM
• Alliance: Ckm^tm2Bew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20440
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A hygroB-polyA cassette inserted into intron 2 of the gene as a result of incorrect gene targeting. Northern blot of skeletal muscle from homozygous mutant animals showed a normal transcript with reduced expression. The protein product exhibits a 3-fold reduction in enzyme activity. (J:20440)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ckm Mutation: 38 strains or lines available

References

• Original: J:20440 van Deursen J, et al., Creatine kinase (CK) in skeletal muscle energy metabolism: a study of mouse mutants with graded reduction in muscle CK expression. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9091-5
• All: 2 reference(s)