Acadl^tm1Uab
Targeted Allele Detail

Summary

• Symbol: Acadl^tm1Uab
• Name: acyl-Coenzyme A dehydrogenase, long-chain; targeted mutation 1, University of Alabama
• MGI ID: MGI:2180079
• Synonyms: LCAD^-
• Gene: Acadl Location: Chr1:66869998-66902436 bp, - strand Genetic Position: Chr1, 33.64 cM
• Alliance: Acadl^tm1Uab page

Histopathology on Acadl^tm1Uab/Acadl⁺ and Acadl^tm1Uab/Acadl^tm1Uab mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51660
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A gap-repair insertion was used to duplicate exons 3 and 4. Analysis of the mutant cDNA showed a duplication of exons 3 and 4 as well as the excision of the neomycin selection gene and the plasmid sequence. Northern blot analysis demonstrated normal expression levels of mutant transcript. No protein was detected by immunoblots in isolates from liver mitochondria of homozygous mutant animals. (J:51660)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Acadl Mutation: 22 strains or lines available

References

• Original: J:51660 Kurtz DM, et al., Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15592-7
• All: 31 reference(s)