Acr^tm1Tba
Targeted Allele Detail

Summary

• Symbol: Acr^tm1Tba
• Name: acrosin prepropeptide; targeted mutation 1, Tadashi Baba
• MGI ID: MGI:2180087
• Gene: Acr Location: Chr15:89452549-89458790 bp, + strand Genetic Position: Chr15, 44.96 cM, cytoband E-F
• Alliance: Acr^tm1Tba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21944
• Parent Cell Line: A3-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was used to replace a portion of exon 2 and all of exon 3. The deleted region encoded His70 and Asp124, which are required for serine protease activity, as well as 5 Cys residues involved in disulfide bonds. Northern analysis using a cDNA fragment containing exons 2 and 3 showed an absence of mRNA in extracts isolated from the testes of homozygous mutant mice. Protease activity in cauda epididymal sperm was assayed and found to be absent in homozygous animals. (J:21944)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Acr Mutation: 49 strains or lines available

References

• Original: J:21944 Baba T, et al., Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. J Biol Chem. 1994 Dec 16;269(50):31845-9
• All: 4 reference(s)