Actb^tm1Bhr
Targeted Allele Detail

Summary

• Symbol: Actb^tm1Bhr
• Name: actin, beta; targeted mutation 1, Richard R Behringer
• MGI ID: MGI:2180089
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm1Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46634
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A promoterless hygromycin-lacZ fusion gene with a splice acceptor was introduced into the first intron of the beta actin locus. The presence of normal transcript in homozygous mutants suggests that the fusion gene is sporadically excised during splicing, resulting in a hypomorphic allele. Expression analysis indicated that the integration event does not result in ubiquitous expression of Beta galactosidase. (J:46634)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:46634 Shawlot W, et al., Restricted beta-galactosidase expression of a hygromycin-lacZ gene targeted to the beta-actin locus and embryonic lethality of beta-actin mutant mice. Transgenic Res. 1998 Mar;7(2):95-103
• All: 2 reference(s)