Nefl^tm1Jpj
Targeted Allele Detail

Summary

• Symbol: Nefl^tm1Jpj
• Name: neurofilament, light polypeptide; targeted mutation 1, Jean-Pierre Julien
• MGI ID: MGI:2180095
• Synonyms: L-, NF-L-, NFL-
• Gene: Nefl Location: Chr14:68321312-68326544 bp, + strand Genetic Position: Chr14, 35.46 cM, cytoband D3
• Alliance: Nefl^tm1Jpj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44421
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 0.7 kb of the gene including exon 1 was replaced with a neomycin resistance cassette by homologous recombination. Northern and Western blots of brain and nervous tissue from homozygous mutant animals did not detect gene expression, nor were any truncated mRNA species or protein fragments detected. (J:44421)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nefl Mutation: 17 strains or lines available

References

• Original: J:44421 Zhu Q, et al., Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp Neurol. 1997 Nov;148(1):299-316
• All: 35 reference(s)