Tle5^tm1Grid
Targeted Allele Detail

Summary

• Symbol: Tle5^tm1Grid
• Name: TLE family member 5, transcriptional modulator; targeted mutation 1, Tom Gridley
• MGI ID: MGI:2180116
• Synonyms: Aes-, Grg5^-, Grg^delta1
• Gene: Tle5 Location: Chr10:81395322-81402196 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tle5^tm1Grid page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29516
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exon 2 and also introduces both termination and polyadenylation signals into the open reading frame. The authors noted that if the aforementioned signals were not recognized, the splicing of exons 1 and 3 would result in frameshift mutation. Western analysis of lysates isolated from the brains and spleens of homozygous mutant mice showed an absence of protein expression. (J:29516)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tle5 Mutation: 19 strains or lines available

References

• Original: J:29516 Mallo M, et al., Protein characterization and targeted disruption of Grg, a mouse gene related to the groucho transcript of the Drosophila Enhancer of split complex. Dev Dyn. 1995 Nov;204(3):338-47
• All: 5 reference(s)