Grk2^tm1Mca
Targeted Allele Detail

Summary

• Symbol: Grk2^tm1Mca
• Name: G protein-coupled receptor kinase 2; targeted mutation 1, Marc Caron
• MGI ID: MGI:2180127
• Synonyms: betaARK1^-, GRK2-
• Gene: Grk2 Location: Chr19:4336029-4356250 bp, - strand Genetic Position: Chr19, 3.98 cM
• Alliance: Grk2^tm1Mca page

Cardiac abnormalities in Grk2^tm1Mca/Grk2^tm1Mca mouse embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36567
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced exons 5 through 8, encoding catalytic subdomain I that contains a portion of an ATP-binding site. The insertion also altered the downstream reading frame. Northern blot analysis of mutant embryos showed a 62% reduction of transcripts in heterozygotes and an absence of transcripts in homozygotes. Western blot analysis showed correlative results. (J:36567)

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grk2 Mutation: 37 strains or lines available

References

• Original: J:36567 Jaber M, et al., Essential role of beta-adrenergic receptor kinase 1 in cardiac development and function. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12974-9
• All: 25 reference(s)