Nefm^tm1Ral
Targeted Allele Detail

Summary

• Symbol: Nefm^tm1Ral
• Name: neurofilament, medium polypeptide; targeted mutation 1, Robert A Lazzarini
• MGI ID: MGI:2180135
• Gene: Nefm Location: Chr14:68356994-68362453 bp, - strand Genetic Position: Chr14, 35.46 cM
• Alliance: Nefm^tm1Ral page

Lumbar root axon abnormalities in Nefm^tm1Ral/Nefm^tm1Ral and Nefh^tm1Ral/Nefh^tm1Ral Nefm^tm1Ral/Nefm^tm1Ral mice, but not Nefh^tm1Ral/Nefh^tm1Ral mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47438
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 1 was disrupted by insertion of a PGK-neo cassette by homologous recombination. RNase protection assays using probes to exon 1 and exon 3 did not detect gene expression in brain of homozygous mutant animals. Western blot analysis and immunohistochemistry of brain sections from homozygous mutant animals confirmed the absence of protein expression. (J:47438)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nefm Mutation: 28 strains or lines available

References

• Original: J:47438 Elder GA, et al., Absence of the mid-sized neurofilament subunit decreases axonal calibers, levels of light neurofilament (NF-L), and neurofilament content. J Cell Biol. 1998 May 4;141(3):727-39
• All: 4 reference(s)