Syt1^tm3Sud
Targeted Allele Detail

Summary

• Symbol: Syt1^tm3Sud
• Name: synaptotagmin I; targeted mutation 3, Thomas C Sudhof
• MGI ID: MGI:2180148
• Synonyms: R233Q, Syt1R233Q+
• Gene: Syt1 Location: Chr10:108333511-108846843 bp, - strand Genetic Position: Chr10, 56.52 cM
• Alliance: Syt1^tm3Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76452
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Insertion of a neomycin resistance cassette into intron 1 and an arginine to glutamine point mutation at position 233 of exon 1 (R233Q) was introduced via homologous recombination. Western blot of brain homogenates from homozygous mutant animals verified the presence of protein expression and enzyme assays demonstrated similar phospholipid- and syntaxin-binding ability compared to wild-type. However, calcium affinity was decreased by 2-fold. (J:76452)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Syt1 Mutation: 41 strains or lines available

References

• Original: J:76452 Fernandez-Chacon R, et al., Synaptotagmin I functions as a calcium regulator of release probability. Nature. 2001 Mar 1;410(6824):41-9
• All: 5 reference(s)