Akt1^tm1Mbb
Targeted Allele Detail

Summary

• Symbol: Akt1^tm1Mbb
• Name: thymoma viral proto-oncogene 1; targeted mutation 1, Morris J Birnbaum
• MGI ID: MGI:2180150
• Synonyms: Akt-
• Gene: Akt1 Location: Chr12:112620260-112641266 bp, - strand Genetic Position: Chr12, 61.2 cM, cytoband F1-F2
• Alliance: Akt1^tm1Mbb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72176
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene was used to replace exons 4 through 7 and the 5' portion of exon 8. Exon 5 is known to encode an lysine residue essential for catalytic activity. Expression was undetected in homozygous mutant animals by both Northern and Western blot analyses. The authors noted that the expression of Akt2 and Akt3 was normal in mice homozygous for the targeted allele. (J:72176)

Disease models

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Akt1^tm1Mbb

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akt1 Mutation: 34 strains or lines available

References

• Original: J:72176 Cho H, et al., Akt1/pkbalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J Biol Chem. 2001 Oct 19;276(42):38349-52
• All: 113 reference(s)