Cacng1^tm1Vfz
Targeted Allele Detail

Summary

• Symbol: Cacng1^tm1Vfz
• Name: calcium channel, voltage-dependent, gamma subunit 1; targeted mutation 1, Veit Flockerzi
• MGI ID: MGI:2180153
• Synonyms: gamma-, gamma1-
• Gene: Cacng1 Location: Chr11:107594044-107607302 bp, - strand Genetic Position: Chr11, 70.29 cM, cytoband E1
• Alliance: Cacng1^tm1Vfz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62133
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3' portion of exon 1 and adjacent splice donor site were replaced with a neo resistance cassette via homologous recombination. Absence of gene expression was confirmed by Northern and Western blot analysis of skeletal muscle from homozygous mutant animals. In addition, RT-PCR failed to amplify the complete coding region of the gene as well as portions of the transcript corresponding to exons 2, 3, and 4. (J:62133)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacng1 Mutation: 14 strains or lines available

References

• Original: J:62133 Freise D, et al., Absence of the gamma subunit of the skeletal muscle dihydropyridine receptor increases L-type Ca2+ currents and alters channel inactivation properties. J Biol Chem. 2000 May 12;275(19):14476-81
• All: 4 reference(s)