Ambp^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Ambp^tm1Miya
• Name: alpha 1 microglobulin/bikunin precursor; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:2180160
• Synonyms: UTI^-
• Gene: Ambp Location: Chr4:63061512-63072409 bp, - strand Genetic Position: Chr4, 33.96 cM, cytoband C1-C3
• Alliance: Ambp^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68118
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a portion of exon 8, all of exon 9, and a portion of exon 10. The deleted region encodes the Kunitz domains that impart serine proteinase inhibitor activity in bikunin. Western blot analysis of plasma from homozygous mutant mice showed an absence of the two forms of the encoded protein (220kDa and 120kDa) that were present in wild-type mice. (J:68118)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ambp Mutation: 25 strains or lines available

References

• Original: J:68118 Sato H, et al., Impaired fertility in female mice lacking urinary trypsin inhibitor. Biochem Biophys Res Commun. 2001 Mar 16;281(5):1154-60
• All: 4 reference(s)