Stambp^tm1Sug
Targeted Allele Detail

Summary

• Symbol: Stambp^tm1Sug
• Name: STAM binding protein; targeted mutation 1, Kazuo Sugamura
• MGI ID: MGI:2180168
• Synonyms: AMSH^-/-
• Gene: Stambp Location: Chr6:83520193-83549711 bp, - strand Genetic Position: Chr6, 35.94 cM
• Alliance: Stambp^tm1Sug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72940
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was inserted 5' to exon 3 and a neomycin selection cassette followed by a loxP site was inserted 3' to exon 4 through homologous recombination. The transient expression of Cre-recombinase within the targeted ES cells resulted in the excision of exons 3 and 4 and the neomycin selection gene. Northern blot analysis of total RNA isolated from the brains of homozygous animals demonstrated that no detectable transcript was expressed from this allele. (J:72940)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stambp Mutation: 24 strains or lines available

References

• Original: J:72940 Ishii N, et al., Loss of Neurons in the Hippocampus and Cerebral Cortex of AMSH-Deficient Mice. Mol Cell Biol. 2001 Dec;21(24):8626-37
• All: 2 reference(s)