Araf^tm1Mmc
Targeted Allele Detail

Summary

• Symbol: Araf^tm1Mmc
• Name: Araf proto-oncogene, serine/threonine kinase; targeted mutation 1, Martin McMahon
• MGI ID: MGI:2180180
• Synonyms: X^-
• Gene: Araf Location: ChrX:20664053-20726758 bp, + strand Genetic Position: ChrX, 16.3 cM, cytoband A2-A3.1
• Alliance: Araf^tm1Mmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33951
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a region encoding the cysteine finger of the CR1 domain, the entire CR2 domain, and the ATP-binding site of the CR3 domain. Western blot analysis of embryonic fibroblasts derived from hemizygous mice indicated an absence of protein encoded by the targeted locis and showed normal levels of protein produced by an adjacent locus. (J:33951)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Araf Mutation: 34 strains or lines available

References

• Original: J:33951 Pritchard CA, et al., Post-natal lethality and neurological and gastrointestinal defects in mice with targeted disruption of the A-Raf protein kinase gene. Curr Biol. 1996 May 1;6(5):614-7
• All: 4 reference(s)