Scn10a^tm1Jwo
Targeted Allele Detail

Summary

• Symbol: Scn10a^tm1Jwo
• Name: sodium channel, voltage-gated, type X, alpha; targeted mutation 1, John N Wood
• MGI ID: MGI:2180183
• Synonyms: Nav1.8^-, Scn10a^tm1Jnw, SNS^-
• Gene: Scn10a Location: Chr9:119437522-119548388 bp, - strand Genetic Position: Chr9, 71.33 cM, cytoband distal
• Alliance: Scn10a^tm1Jwo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76456
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 4 and all of exon 5, which encodes the S4 voltage sensor of domain I, were replaced with a PGK-neo cassette via homologous recombination. The PGK-neo cassette introduces stop codons in all 3 reading frames. RT-PCR analysis detected weak expression of mutant transcripts in homozygous mutant animals. (J:76456)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Scn10a Mutation: 102 strains or lines available

References

• Original: J:76456 Akopian AN, et al., The tetrodotoxin-resistant sodium channel SNS has a specialized function in pain pathways. Nat Neurosci. 1999 Jun;2(6):541-8
• All: 28 reference(s)