Fer^tm1Pag
Targeted Allele Detail

Summary

• Symbol: Fer^tm1Pag
• Name: FER tyrosine kinase; targeted mutation 1, Peter A Greer
• MGI ID: MGI:2180299
• Synonyms: fer^D743R, fer^DR
• Gene: Fer Location: Chr17:64170057-64446491 bp, + strand Genetic Position: Chr17, 33.06 cM
• Alliance: Fer^tm1Pag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67350
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An aspartate to arginine mutation at position 743 (D743R) was introduced into exon 19 via homologous recombination in conjunction with a neo resistance cassette downstream of exon 20. The mutation is designed to inactivate the kinase domain by destabilizing the catalytic loop. Western blot analysis of homozygous mutant animals detected protein expression in testis but greatly reduced protein expression in liver. Kinase assays demonstrated complete absence of kinase activity in both testis and liver extracts from homozygous mutants. (J:67350)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fer Mutation: 36 strains or lines available

References

• Original: J:67350 Craig AW, et al., Mice devoid of fer protein-tyrosine kinase activity are viable and fertile but display reduced cortactin phosphorylation. Mol Cell Biol. 2001 Jan;21(2):603-13
• All: 11 reference(s)