Asgr1^tm1Ishi
Targeted Allele Detail

Summary

• Symbol: Asgr1^tm1Ishi
• Name: asialoglycoprotein receptor 1; targeted mutation 1, Shun Ishibashi
• MGI ID: MGI:2180369
• Synonyms: MHL-1 -
• Gene: Asgr1 Location: Chr11:69944911-69948720 bp, + strand Genetic Position: Chr11, 42.98 cM
• Alliance: Asgr1^tm1Ishi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68876
• Parent Cell Line: JH1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene under the control of a pol2 promoter replaced the transcription initiation codon and the sequences encoding the transmembrane domain. Northern blot analysis showed an absence of normal transcipt in homozygous mutant mice. A truncated message was detected, however, Western blot analysis showed there to be no protein expression in homozygous mutant animals. (J:68876)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Asgr1 Mutation: 16 strains or lines available

References

• Original: J:68876 Tozawa R, et al., Asialoglycoprotein receptor deficiency in mice lacking the major receptor subunit. Its obligate requirement for the stable expression of oligomeric receptor. J Biol Chem. 2001 Apr 20;276(16):12624-8
• All: 3 reference(s)