Atf4^tm1Jml
Targeted Allele Detail

Summary

• Symbol: Atf4^tm1Jml
• Name: activating transcription factor 4; targeted mutation 1, Jeffrey M Leiden
• MGI ID: MGI:2180371
• Synonyms: CREB-2^-
• Gene: Atf4 Location: Chr15:80139385-80141742 bp, + strand Genetic Position: Chr15, 37.85 cM
• Alliance: Atf4^tm1Jml page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62578
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region was replaced with a targeting construct containing a neomycin gene under the control of a PGK promoter. Northern blot analysis using a probe specific to exon 3 showed a lack of message in homozygous mutant mice, while Western blot analysis did not detect protein expression. (J:62578)

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atf4 Mutation: 22 strains or lines available

References

• Original: J:62578 Hettmann T, et al., Microphthalmia due to p53-mediated apoptosis of anterior lens epithelial cells in mice lacking the CREB-2 transcription factor. Dev Biol. 2000 Jun 1;222(1):110-23
• All: 12 reference(s)