Atf4^tm1Tow
Targeted Allele Detail

Summary

• Symbol: Atf4^tm1Tow
• Name: activating transcription factor 4; targeted mutation 1, Timothy Townes
• MGI ID: MGI:2180373
• Synonyms: Atf4^-, Atf4 KO, Atf4^tm1Tmt
• Gene: Atf4 Location: Chr15:80139385-80141742 bp, + strand Genetic Position: Chr15, 37.85 cM
• Alliance: Atf4^tm1Tow page

Atf4^tm1Tow/Atf4^tm1Tow mice show pancreatic hypotrophy

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74479
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3, comprising the entire coding region, were replaced with a targeting construct containing a neomycin resistance gene. Expression analysis confirmed the absence of transcript in homozygous mutant mice. (J:74479)

Expression

In Mice Carrying this Mutation:	19 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atf4 Mutation: 22 strains or lines available

Notes

ES cell line = HM1 (129P2/OlaHsd-Hprt^b-m3) or R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺).

The data presented in J:74479 were obtained using R1 ES cells, though the authors report that they also generated mice with targeted alleles using HM1 ES cells.

References

• Original: J:74479 Masuoka HC, et al., Targeted disruption of the activating transcription factor 4 gene results in severe fetal anemia in mice. Blood. 2002 Feb 1;99(3):736-45
• All: 41 reference(s)