Atp2a3^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Atp2a3^tm1Ges
• Name: ATPase, Ca++ transporting, ubiquitous; targeted mutation 1, Gary E Shull
• MGI ID: MGI:2180376
• Synonyms: SERCA3^-
• Gene: Atp2a3 Location: Chr11:72851995-72883869 bp, + strand Genetic Position: Chr11, 45.0 cM
• Alliance: Atp2a3^tm1Ges page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44333
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 5, which encodes amino acids 109 through 154. Northern blot analysis of RNA isolated from colon, lung, and aorta tissue showed the presence of a shortened transcript in homozygous mutant mice. Sequence analysis of the mutant message identified a cryptic donor site in exon 5 that led to the excision of 84 nucleotides encoding amino acids 127 through 154. Transfection of microsomes, showed that the mutant protein product was incapable of calcium-uptake. (J:44333)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atp2a3 Mutation: 35 strains or lines available

References

• Original: J:44333 Liu LH, et al., Defective endothelium-dependent relaxation of vascular smooth muscle and endothelial cell Ca2+ signaling in mice lacking sarco(endo)plasmic reticulum Ca2+-ATPase isoform 3. J Biol Chem. 1997 Nov 28;272(48):30538-45
• All: 6 reference(s)