Sim1^tm1Fan
Targeted Allele Detail

Summary

• Symbol: Sim1^tm1Fan
• Name: single-minded family bHLH transcription factor 1; targeted mutation 1, Chen-Ming Fan
• MGI ID: MGI:2180390
• Synonyms: Sim^-
• Gene: Sim1 Location: Chr10:50770850-50865248 bp, + strand Genetic Position: Chr10, 24.87 cM, cytoband B3-B4
• Alliance: Sim1^tm1Fan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50772
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette was inserted to replace the first 750 nucleotides of the 5' region, the initiation codon, and the first 17 codons of the basic helix-loop-helix (bHLH) domain. While message was present in homozygous mutant mice at levels only slightly below normal, immunocytochemistry analysis showed an absence of protein product in the coronal sections of the brain. (J:50772)

Disease models

Expression

In Mice Carrying this Mutation:	29 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sim1 Mutation: 52 strains or lines available

References

• Original: J:50772 Michaud JL, et al., Development of neuroendocrine lineages requires the bHLH-PAS transcription factor SIM1. Genes Dev. 1998 Oct 15;12(20):3264-75
• All: 11 reference(s)