Npas2^tm1Slm
Targeted Allele Detail

Summary

• Symbol: Npas2^tm1Slm
• Name: neuronal PAS domain protein 2; targeted mutation 1, Steven L McKnight
• MGI ID: MGI:2180392
• Synonyms: Npas2^-, NPAS2-lacZ, NPAS-deltabHLH
• Gene: Npas2 Location: Chr1:39233013-39402321 bp, + strand Genetic Position: Chr1, 17.98 cM
• Alliance: Npas2^tm1Slm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63035
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ-neo fusion gene was inserted into exon 2, which encodes the basic helix-loop-helix (bHLH) domain. Northern blot analysis using a bHLH probe failed to detect transcript in total RNA isolated from homozygous mutant brain tissue. A second transcript, in which the inserted lacZ-neo gene is excised, was observed. This transcript was also found to be functionally inactive due to the lack of the bHLH domain. (J:63035)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Npas2 Mutation: 45 strains or lines available

References

• Original: J:63035 Garcia JA, et al., Impaired cued and contextual memory in NPAS2-deficient mice. Science. 2000 Jun 23;288(5474):2226-30
• All: 24 reference(s)