Cd247^tm1Mhu
Targeted Allele Detail

Summary

• Symbol: Cd247^tm1Mhu
• Name: CD247 antigen; targeted mutation 1, Manley Huang
• MGI ID: MGI:2180467
• Synonyms: CD3-zeta^-
• Gene: Cd247 Location: Chr1:165616250-165704846 bp, + strand Genetic Position: Chr1, 73.14 cM, cytoband G-H
• Alliance: Cd247^tm1Mhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:15579
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neo cassette was introduced into exon 2 via homologous recombination thus disrupting the reading frame of the gene. Absence of gene expression in homozygous mutant mice was verified by Western blot analysis of spleen and intestinal intraepithelial cells. (J:15579)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd247 Mutation: 11 strains or lines available

References

• Original: J:15579 Liu CP, et al., Abnormal T cell development in CD3-zeta-/- mutant mice and identification of a novel T cell population in the intestine. EMBO J. 1993 Dec;12(12):4863-75
• All: 9 reference(s)