Cd247^tm1Tks
Targeted Allele Detail

Summary

• Symbol: Cd247^tm1Tks
• Name: CD247 antigen; targeted mutation 1, Takashi Saito
• MGI ID: MGI:2180484
• Synonyms: CD3zeta^-, zetaKO, zetaT
• Gene: Cd247 Location: Chr1:165616250-165704846 bp, + strand Genetic Position: Chr1, 73.14 cM, cytoband G-H
• Alliance: Cd247^tm1Tks page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:15271
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.2 kb region containing exon 8 and a splice acceptor sequence of intron 7 was replaced with a neomycin resistance gene via homologous recombination resulting in specific disruption of the zeta isoform while preserving the eta isoform. Absence of expression from the zeta isoform in homozygous mutants was confirmed by RNase protection assay. Interestingly, eta isoform expression in homozygous mutants increased to a level similar to wild-type zeta expression. (J:15271)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd247 Mutation: 11 strains or lines available

References

• Original: J:15271 Ohno H, et al., Developmental and functional impairment of T cells in mice lacking CD3 zeta chains. EMBO J. 1993 Nov;12(11):4357-66
• All: 4 reference(s)