Bhlhe40^tm1Tan
Targeted Allele Detail

Summary

• Symbol: Bhlhe40^tm1Tan
• Name: basic helix-loop-helix family, member e40; targeted mutation 1, Reshma Taneja
• MGI ID: MGI:2180488
• Synonyms: Dec1^-, S2^-, Stra13^-
• Gene: Bhlhe40 Location: Chr6:108637590-108643886 bp, + strand Genetic Position: Chr6, 49.85 cM, cytoband E-F1
• Alliance: Bhlhe40^tm1Tan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72651
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene under the control of a GTI-II promoter replaced a region encoding the first 6 amino acids, including the initiator-methionine, and a portion of the proximal promoter. Northern blot analyis confirmed a lack of transcript in heart, liver, and kidney tissue obtained from homozygous mutant mice. An absence of protein expression was determined homozygous mutant MEFs via Western blot analysis. (J:72651)

Expression

In Mice Carrying this Mutation:	1 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bhlhe40 Mutation: 51 strains or lines available

References

• Original: J:72651 Sun H, et al., Defective T cell activation and autoimmune disorder in Stra13-deficient mice. Nat Immunol. 2001 Nov;2(11):1040-7
• All: 25 reference(s)