Mtr^tm1Lcb
Targeted Allele Detail

Summary

• Symbol: Mtr^tm1Lcb
• Name: 5-methyltetrahydrofolate-homocysteine methyltransferase; targeted mutation 1, Lawrence C Brody
• MGI ID: MGI:2180496
• Synonyms: MS^-, Mtr-
• Gene: Mtr Location: Chr13:12197598-12272999 bp, - strand Genetic Position: Chr13, 4.52 cM
• Alliance: Mtr^tm1Lcb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67384
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A PGK-neo cassette was inserted to delete a region spanning from the middle of exon 3 through exon 6. This deleted region encodes the essential homocysteine binding domain. Reduced levels of transcript were observed in extracts from the liver and kidney of heterozygous mutant mice. Enzyme activity levels in heterozygotes were found to be approximately 60% of wild-type. (J:67384)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtr Mutation: 50 strains or lines available

References

• Original: J:67384 Swanson DA, et al., Targeted disruption of the methionine synthase gene in mice. Mol Cell Biol. 2001 Feb;21(4):1058-65
• All: 3 reference(s)