Bub3^tm1Khac
Targeted Allele Detail

Summary

• Symbol: Bub3^tm1Khac
• Name: BUB3 mitotic checkpoint protein; targeted mutation 1, K H Andy Choo
• MGI ID: MGI:2180502
• Synonyms: Bub3-
• Gene: Bub3 Location: Chr7:131162081-131173625 bp, + strand Genetic Position: Chr7, 74.36 cM
• Alliance: Bub3^tm1Khac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64874
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 were replaced with a lacZ-neomycin fusion gene lacking a promoter but possessing a splice acceptor site and an internal ribosome entry site (IRES). The authors believed that only the first 65 amino acids would be translated, producing a protein lacking three of the four WD40 domains as well as the protein-interacting kinetochore domain. (J:64874)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bub3 Mutation: 23 strains or lines available

Notes

ES cell line = W9.5 (129S1/Sv-p⁺ Tyr⁺ Kitl⁺) or 129/1 (129P2/OlaHsd)

References

• Original: J:64874 Kalitsis P, et al., Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 2000 Sep 15;14(18):2277-82
• All: 2 reference(s)