Cbl^tm1Ddlb
Targeted Allele Detail

Summary

• Symbol: Cbl^tm1Ddlb
• Name: Casitas B-lineage lymphoma; targeted mutation 1, David D L Bowtell
• MGI ID: MGI:2180578
• Synonyms: c-Cbl^-
• Gene: Cbl Location: Chr9:44054273-44145346 bp, - strand Genetic Position: Chr9, 24.72 cM
• Alliance: Cbl^tm1Ddlb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76895
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a PGK-neo cassette disrupted the exon encoding the phosphotyrosine binding (PTB) domain. Western blot analysis confirmed an absence of normal protein product in homozygous mutant mice. The presence of a truncated protein was noted and determined to be the result of an in-frame splicing event in which the targeted exon was deleted, thus all peptides produced from targeted alleles lacked the conserved residues within the PTB domain. (J:76895)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Cbl Mutation: 59 strains or lines available

References

• Original: J:76895 Murphy MA, et al., Tissue hyperplasia and enhanced T-cell signalling via ZAP-70 in c-Cbl-deficient mice. Mol Cell Biol. 1998 Aug;18(8):4872-82
• All: 18 reference(s)