Ctla4^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Ctla4^tm1Mak
• Name: cytotoxic T-lymphocyte-associated protein 4; targeted mutation 1, Tak W Mak
• MGI ID: MGI:2180669
• Synonyms: Ctla-4^-
• Gene: Ctla4 Location: Chr1:60948184-60954991 bp, + strand Genetic Position: Chr1, 30.58 cM, cytoband C
• Alliance: Ctla4^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29652
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection gene replaced exon 2 and the 5' portion of exon 3. A lack of expression in homozygous mutant mice was demonstrated via fluorescence-activated cell sorting (FACS) analysis. (J:29652)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ctla4 Mutation: 54 strains or lines available

Notes

Homozygous mutant mice accumulate T cell blasts in the lymph nodes and spleens which also infiltrate liver, heart, lung, and pancreas. Serum immunoglobulin is elevated, and the mice die by 3 to 4 weeks old. T cells proliferate when stimulated via T cell receptors, but are liable to Fas induced apoptosis (J:29652).

References

• Original: J:29652 Waterhouse P, et al., Lymphoproliferative disorders with early lethality in mice deficient in Ctla-4 [see comments]. Science. 1995 Nov 10;270(5238):985-8
• All: 17 reference(s)