Ccr7^tm1Rfor
Targeted Allele Detail

Summary

• Symbol: Ccr7^tm1Rfor
• Name: C-C motif chemokine receptor 7; targeted mutation 1, Reinhold Forster
• MGI ID: MGI:2180679
• Synonyms: CCR7-
• Gene: Ccr7 Location: Chr11:99035025-99045903 bp, - strand Genetic Position: Chr11, 62.92 cM, cytoband D
• Alliance: Ccr7^tm1Rfor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57976
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: 0.5 kb of exon 3 encoding serine 139 to aspartic acid 309 was disrupted by insertion of a neomycin resistance cassette via homologous recombination. Successful targeting was verified in homozygous mutant animals by Southern blot analysis and chemotaxis assays. Spleen cells from homozygous animals demonstrated lack of chemotactic response towards EBI-1 ligand chemokine (ELC), a known ligand of the targeted gene. (J:57976)

Disease models

Expression

In Structures Affected by this Mutation:

18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ccr7 Mutation: 29 strains or lines available

References

• Original: J:57976 Forster R, et al., CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell. 1999 Oct 1;99(1):23-33
• All: 230 reference(s)