Aldh1a2^tm1Gdu
Targeted Allele Detail

Summary

• Symbol: Aldh1a2^tm1Gdu
• Name: aldehyde dehydrogenase family 1, subfamily A2; targeted mutation 1, Gregg Duester
• MGI ID: MGI:2180721
• Synonyms: Raldh2^-
• Gene: Aldh1a2 Location: Chr9:71123071-71203525 bp, + strand Genetic Position: Chr9, 39.85 cM
• Alliance: Aldh1a2^tm1Gdu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75950
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 are deleted and are replaced by a PGK-hygromycin selectable marker. This prevents translation beyond residue 67 and deletes the entire catalytic domain (residues 271-484) including the catalytic cysteine at residue 301. Whole mount immunohistochemistry experiments confirmed that the encoded protein was undetectable in homozygous embryos. (J:75950)

Expression

In Mice Carrying this Mutation:	110 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Aldh1a2 Mutation: 38 strains or lines available

References

• Original: J:75950 Mic FA, et al., Novel retinoic acid generating activities in the neural tube and heart identified by conditional rescue of Raldh2 null mutant mice. Development. 2002 May;129(9):2271-82
• All: 32 reference(s)