Cd34^tm1Lal
Targeted Allele Detail

Summary

• Symbol: Cd34^tm1Lal
• Name: CD34 antigen; targeted mutation 1, Larry A Lasky
• MGI ID: MGI:2180758
• Gene: Cd34 Location: Chr1:194621239-194643587 bp, + strand Genetic Position: Chr1, 98.38 cM
• Alliance: Cd34^tm1Lal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30621
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into the exon encoding the signal sequence, disrupting the reading frame downstream. Homozygosity in ES cells was achieved by gene conversion. FACS analysis of homozygous mutant ES cells showed an absence of protein surface expression. The absence of encoded protein mice derived from these clones was determined via immunohistochemical analysis. (J:30621)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd34 Mutation: 33 strains or lines available

References

• Original: J:30621 Cheng J, et al., Hematopoietic defects in mice lacking the sialomucin CD34. Blood. 1996 Jan 15;87(2):479-90
• All: 1 reference(s)