Limk2^tm1.1Tnak
Targeted Allele Detail

Summary

• Symbol: Limk2^tm1.1Tnak
• Name: LIM domain kinase 2; targeted mutation 1.1, Toshikazu Nakamura
• MGI ID: MGI:2180767
• Synonyms: Limk2-
• Gene: Limk2 Location: Chr11:3294256-3359189 bp, - strand Genetic Position: Chr11, 2.34 cM
• Alliance: Limk2^tm1.1Tnak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73966
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele is a derivative of Limk2^tm1Tnak. Cre-mediated recombination in vivo under control of the ACTB promoter excised the inserted neo cassette and exons 3, 4, 5, and 1t in the germline. Exons 3-5 encode the second LIM domain and part of the PDZ domain. Exon 1t encodes the initiation exon for the testis specific isoform of the protein. Western blot analysis did not detect protein in fibroblast cells derived from homozygous mutant embryos. Northern blot analysis of testis did not detect any mRNA in homozygous mutant mice. (J:73966)

Expression

In Mice Carrying this Mutation:	61 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Limk2 Mutation: 78 strains or lines available

References

• Original: J:73966 Takahashi H, et al., Impaired spermatogenic ability of testicular germ cells in mice deficient in the LIM-kinase 2 gene. Dev Biol. 2002 Jan 15;241(2):259-72
• All: 6 reference(s)