Cdc45^tm1Dta
Targeted Allele Detail

Summary

• Symbol: Cdc45^tm1Dta
• Name: cell division cycle 45; targeted mutation 1, Anindya Dutta
• MGI ID: MGI:2180780
• Synonyms: CDC45^-
• Gene: Cdc45 Location: Chr16:18599197-18630737 bp, - strand Genetic Position: Chr16, 11.63 cM
• Alliance: Cdc45^tm1Dta page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70008
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection gene replaced exons 1 and 2, which encode the 199 amino-terminal residues. RT-PCR analysis of total RNA obtained from heterozygous mutant embryos showed that the level of normal transcript was approximately half of what was detected in wild-type embryos. (J:70008)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdc45 Mutation: 43 strains or lines available

References

• Original: J:70008 Yoshida K, et al., Requirement of CDC45 for postimplantation mouse development. Mol Cell Biol. 2001 Jul;21(14):4598-603
• All: 2 reference(s)