Mthfd2^tm1Rmk
Targeted Allele Detail

Summary

• Symbol: Mthfd2^tm1Rmk
• Name: methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; targeted mutation 1, Robert E MacKenzie
• MGI ID: MGI:2180800
• Synonyms: nmdmc^-, NMDMC-null
• Gene: Mthfd2 Location: Chr6:83282673-83302890 bp, - strand Genetic Position: Chr6, 35.94 cM
• Alliance: Mthfd2^tm1Rmk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76786
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced part of exon 3, exons 4-7, and part of exon 8 via homologous recombination. Homozygous mutant embryos were detected by PCR and Southern blot analysis. (J:76786)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mthfd2 Mutation: 25 strains or lines available

References

• Original: J:76786 Di Pietro E, et al., Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is essential for embryonic development. Mol Cell Biol. 2002 Jun;22(12):4158-66
• All: 1 reference(s)