Cd7^tm1Bfh
Targeted Allele Detail

Summary

• Symbol: Cd7^tm1Bfh
• Name: CD7 antigen; targeted mutation 1, Barton F Haynes
• MGI ID: MGI:2180804
• Synonyms: CD7-
• Gene: Cd7 Location: Chr11:120927573-120930244 bp, - strand Genetic Position: Chr11, 84.85 cM
• Alliance: Cd7^tm1Bfh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48758
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3 was disrupted by the introduction of a stop codon upstream of sequence encoding the transmembrane domain. A PGK-neo cassette was inserted just downstream of the introduced stop codon. Northern blot analysis of thymocytes and splenocytes showed a lack of protein expression in homozygous mutant mice. These results were confirmed by RT-PCR analysis. (J:48758)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd7 Mutation: 15 strains or lines available

References

• Original: J:48758 Lee DM, et al., Immunologic characterization of CD7-deficient mice. J Immunol. 1998 Jun 15;160(12):5749-56
• All: 5 reference(s)