Nhlh1^tm1Thbr
Targeted Allele Detail

Summary

• Symbol: Nhlh1^tm1Thbr
• Name: nescient helix loop helix 1; targeted mutation 1, Thomas Braun
• MGI ID: MGI:2180862
• Synonyms: NSCL-1-
• Gene: Nhlh1 Location: Chr1:171879859-171885140 bp, - strand Genetic Position: Chr1, 79.54 cM
• Alliance: Nhlh1^tm1Thbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73967
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ-neomycin resistance cassette replaced the most of the second exon including almost the entire DNA binding bHLH region and most of the coding region, and placed lacZ under control of endogenous transcriptional elements. RT-PCR analysis of brain did not detect transcript in homozygous mutant E18.5 embryos. Beta-galactosidase was expresssed under the control of the endogenous promoter. (J:73967)

Expression

In Mice Carrying this Mutation:

101 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nhlh1 Mutation: 17 strains or lines available

References

• Original: J:73967 Kruger M, et al., The neuronal basic helix-loop-helix transcription factor NSCL-1 is dispensable for normal neuronal development. Mol Cell Biol. 2002 Feb;22(3):792-800
• All: 3 reference(s)