Sele^tm1Roml
Targeted Allele Detail

Summary

• Symbol: Sele^tm1Roml
• Name: selectin, endothelial cell; targeted mutation 1, Mark A Labow
• MGI ID: MGI:2180868
• Synonyms: E-selectin (-)
• Gene: Sele Location: Chr1:163875773-163885246 bp, + strand Genetic Position: Chr1, 71.35 cM
• Alliance: Sele^tm1Roml page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:22062
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a neomycin resistance cassette into exon 1 after amino acid 162 of the LEC domain resulting in disruption of the protein's carbohydrate-binding domain. Northern blot analysis of lung from homozygous mutant animals detected several alternatively spliced mutant transcripts. Western blot, immunoprecipitation, and immunohistochemistry analyses were unable to detect gene expression in hearts from homozygous mutant animals. (J:22062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sele Mutation: 51 strains or lines available

References

• Original: J:22062 Labow MA, et al., Characterization of E-selectin-deficient mice: demonstration of overlapping function of the endothelial selectins. Immunity. 1994 Nov;1(8):709-20
• All: 7 reference(s)