Cenpa^tm1Khac
Targeted Allele Detail

Summary

• Symbol: Cenpa^tm1Khac
• Name: centromere protein A; targeted mutation 1, K H Andy Choo
• MGI ID: MGI:2180885
• Gene: Cenpa Location: Chr5:30824214-30832181 bp, + strand Genetic Position: Chr5, 16.76 cM
• Alliance: Cenpa^tm1Khac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60186
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a lacZ and neo fusion gene, as well as a splice-acceptor site and internal ribosome-entry site (IRES), replaced exon 2. The deleted region encoded the centromere targeting domain comprised of amino acids 29 through 64. Immunofluorescence staining using an antibody raised against the endogenous protein showed a near absence of protein, as weak signals were observed in some cells, in homozygous mutant embryos. (J:60186)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpa Mutation: 17 strains or lines available

References

• Original: J:60186 Howman EV, et al., Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1148-53
• All: 2 reference(s)