Eci1^tm1Wst
Targeted Allele Detail

Summary

• Symbol: Eci1^tm1Wst
• Name: enoyl-Coenzyme A delta isomerase 1; targeted mutation 1, Wilhelm Stoffel
• MGI ID: MGI:2180887
• Synonyms: eci^-
• Gene: Eci1 Location: Chr17:24645657-24658290 bp, + strand Genetic Position: Chr17, 12.39 cM, cytoband A3.3
• Alliance: Eci1^tm1Wst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76763
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a neomycin cassette into exon 4 via homologous recombination. Northern blot analysis of various tissues from homozygous animals detected very low levels of mutant mRNA indicating transcript instability. Western blot and enzyme analysis did not detect protein product or activity. (J:76763)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eci1 Mutation: 17 strains or lines available

References

• Original: J:76763 Janssen U, et al., Disruption of mitochondrial beta -oxidation of unsaturated fatty acids in the 3,2-trans-enoyl-CoA isomerase-deficient mouse. J Biol Chem. 2002 May 31;277(22):19579-84
• All: 2 reference(s)