Slc12a5^tm1Tjj
Targeted Allele Detail

Summary

• Symbol: Slc12a5^tm1Tjj
• Name: solute carrier family 12, member 5; targeted mutation 1, Thomas J Jentsch
• MGI ID: MGI:2181025
• Synonyms: Kcc2^-
• Gene: Slc12a5 Location: Chr2:164802766-164841651 bp, + strand Genetic Position: Chr2, 85.28 cM, cytoband G2-G3
• Alliance: Slc12a5^tm1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69625
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was replaced with a neomycin selection cassette, introducing a frameshift mutation. The deleted region encoded a portion of the second transmembrane domain. Northern analysis revealed a lack of transcript in homozygous mutant mice. Absence of a truncated protein product was confirmed by Western blot analysis using an antibody raised against the N-terminal. (J:69625)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc12a5 Mutation: 45 strains or lines available

References

• Original: J:69625 Hubner CA, et al., Disruption of KCC2 Reveals an Essential Role of K-Cl Cotransport Already in Early Synaptic Inhibition. Neuron. 2001 May;30(2):515-24
• All: 3 reference(s)