Cldn11^tm1Ral
Targeted Allele Detail

Summary

• Symbol: Cldn11^tm1Ral
• Name: claudin 11; targeted mutation 1, Robert A Lazzarini
• MGI ID: MGI:2181029
• Synonyms: OSP/claudin-11 (-)
• Gene: Cldn11 Location: Chr3:31204069-31218473 bp, + strand Genetic Position: Chr3, 15.14 cM
• Alliance: Cldn11^tm1Ral page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59025
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a promoterless lacZ gene and a floxed PGK-neomycin resistance gene replaced exon 1 and a portion of intron 1. Northern blot analysis of extracts from brains and testes showed a lack of transcript in homozygous mutant mice. Western blot analysis confirmed an absence of encoded protein in brain homogenates. Expression of the inserted lacZ gene was under the control of the endogenous promoter. (J:59025)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Cldn11^tm1Ral/Cldn11^tm1Ral	Not Specified

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms Sex:
behavior/neurological	√	√
tremors	√	√
impaired coordination	√	√
abnormal grip strength	√	√
cellular		√
azoospermia		√
endocrine/exocrine glands		√
abnormal seminiferous tubule morphology		√
abnormal Sertoli cell barrier morphology		√
small testis		√
hearing/vestibular/ear	√	√
cochlear outer hair cell degeneration	√	√
absent strial basal cell tight junctions	√	√
decreased endocochlear potential	√	√
abnormal auditory brainstem response waveform shape	√	√
increased or absent threshold for auditory brainstem response	√	√
abnormal distortion product otoacoustic emission	√	√
deafness	√	√
nervous system	√	√
nervous system phenotype	N	N
cochlear outer hair cell degeneration	√	√
abnormal myelin sheath morphology	√	√
reproductive system		√
abnormal seminiferous tubule morphology		√
abnormal Sertoli cell barrier morphology		√
small testis		√
abnormal spermatogenesis		√
azoospermia		√
male infertility		√
vision/eye	√	√
abnormal visual evoked potential	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	12 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cldn11 Mutation: 15 strains or lines available

References

• Original: J:59025 Gow A, et al., CNS myelin and sertoli cell tight junction strands are absent in Osp/claudin-11 null mice. Cell. 1999 Dec 10;99(6):649-59
• All: 8 reference(s)