Coil^tm1Agm
Targeted Allele Detail

Summary

• Symbol: Coil^tm1Agm
• Name: coilin; targeted mutation 1, A Gregory Matera
• MGI ID: MGI:2181054
• Gene: Coil Location: Chr11:88864761-88882439 bp, + strand Genetic Position: Chr11, 54.34 cM, cytoband D
• Alliance: Coil^tm1Agm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70593
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 10-kb region spanning exon 2 through the stop codon in exon 7 was replaced by a neomycin selection cassette. The deleted region encoded the nuclear localization signals, serine patches, and a conserved carboxyl terminus. Northern blot analysis using probes corresponding to regions outside of the deleted interval showed the presence of a hybrid mRNA. Sequence analysis of the aberrant transcript showed a cryptic splice junction between exon 1 and the inserted neo gene. Western blot analysis using an antibody raised against the carboxyl terminus showed a lack of normal protein, but could not confirm the absence of an 82 residue peptide putatively translated from the exon 1 fragment. Attempts to generate an antibody for the largely hydrophobic amino terminus were not successful. (J:70593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Coil Mutation: 186 strains or lines available

References

• Original: J:70593 Tucker KE, et al., Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product. J Cell Biol. 2001 Jul 23;154(2):293-307
• All: 2 reference(s)