Ptprc^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Ptprc^tm1Mak
• Name: protein tyrosine phosphatase receptor type C; targeted mutation 1, Tak W Mak
• MGI ID: MGI:2181288
• Synonyms: cd45-, CD45-exon6-
• Gene: Ptprc Location: Chr1:137990599-138103446 bp, - strand Genetic Position: Chr1, 60.73 cM
• Alliance: Ptprc^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:13163
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 6, which is excised in some isoforms, was deleted and replaced with a neomycin selection cassette. Immunofluorescence analysis, using an antibody raised against all isoforms, of cells isolated from homozygous mutant mice showed an absence of encoded protein in B lymphocytes and low levels of expression in peripheral T cells. Flow cytometric analysis of peripheral T cells confirmed that protein containing the region encoded by exon 6 was absent, while protein containing the region encoded by exon 5 was present at low levels. (J:13163)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ptprc Mutation: 189 strains or lines available

Notes

ES cell line = D3NS.

References

• Original: J:13163 Kishihara K, et al., Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 1993 Jul 16;74(1):143-56
• All: 44 reference(s)