Lum^tm1Wwk
Targeted Allele Detail

Summary

• Symbol: Lum^tm1Wwk
• Name: lumican; targeted mutation 1, Winston W Y Kao
• MGI ID: MGI:2181377
• Synonyms: lum-, Lum^-
• Gene: Lum Location: Chr10:97401363-97408565 bp, + strand Genetic Position: Chr10, 50.32 cM
• Alliance: Lum^tm1Wwk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60203
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of 481 bp of exon 2 with a PGK-hprt cassette (in the opposite orientation) via homologous recombination. Northern blot, in situ hybridization, and immunohistochemistry confirmed the absence of mRNA and protein expression in eyes of homozygous mutant animals. (J:60203)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lum Mutation: 22 strains or lines available

References

• Original: J:60203 Saika S, et al., Role of lumican in the corneal epithelium during wound healing. J Biol Chem. 2000 Jan 28;275(4):2607-12
• All: 16 reference(s)