Nr1h3^tm1Djm
Targeted Allele Detail

Summary

• Symbol: Nr1h3^tm1Djm
• Name: nuclear receptor subfamily 1, group H, member 3; targeted mutation 1, David J Mangelsdorf
• MGI ID: MGI:2181380
• Synonyms: LXRalpha (-), Nr1h3^-
• Gene: Nr1h3 Location: Chr2:91014406-91033179 bp, - strand Genetic Position: Chr2, 50.52 cM
• Alliance: Nr1h3^tm1Djm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47971
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3-6 encoding the DNA-binding and ligand-binding domains (amino acids 87-327) were replaced with a neomycin resistance gene via homologous recombination. Northern blot and Western blot analysis of liver from homozygous mutant animals verified the absence of mRNA and protein expression. (J:47971)

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Nr1h3 Mutation: 32 strains or lines available

References

• Original: J:47971 Peet DJ, et al., Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR alpha. Cell. 1998 May 29;93(5):693-704
• All: 111 reference(s)