Cyp27a1^tm1Elt
Targeted Allele Detail

Summary

• Symbol: Cyp27a1^tm1Elt
• Name: cytochrome P450, family 27, subfamily a, polypeptide 1; targeted mutation 1, Eran Leitersdorf
• MGI ID: MGI:2181406
• Synonyms: cyp27^-, Cyp27 -, Cyp27a1^-
• Gene: Cyp27a1 Location: Chr1:74752733-74777051 bp, + strand Genetic Position: Chr1, 38.54 cM, cytoband C3
• Alliance: Cyp27a1^tm1Elt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48127
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 71 bp fragment of exon 8 was deleted and replaced with a neomycin resistance gene via homologous recombination. The mutant allele was verified by Northern blot analysis of liver extracts from homozygous mutant mice. Western blot analysis of total liver mitochondrial protein from homozygous mutants confirmed the absence of protein product. (J:48127)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp27a1 Mutation: 30 strains or lines available

References

• Original: J:48127 Rosen H, et al., Markedly reduced bile acid synthesis but maintained levels of cholesterol and vitamin D metabolites in mice with disrupted sterol 27-hydroxylase gene. J Biol Chem. 1998 Jun 12;273(24):14805-12
• All: 34 reference(s)